Background: Hoechst 33342 is hoechst a specific fluorescent DNA stain that binds within the minor groove of double- stranded AT- rich regions. HoechstNone None None None EU OEL ( STEL) EU Skin Notation Chemical Name Austria Denmark Poland Switzerland HoechstNone HoechstNone None None None None None Handling Chemical Name Ireland Norway Spain hoechst OEL ( TWA) HoechstNone None datasheet None Avoid contact with skin eyes datasheet clothing. Biochim Biophys Acta 949, 158- 68. On binding to DNA, the fluorescence greatly increases. The compound is excited by ultraviolet light at around 350 nm emits blue/ cyan fluorescence light around an emission maximum at 461 nm. 93) that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT- rich DNA sequences. 1 datasheet It emits datasheet blue fluorescence ( datasheet excitation 350 nm/ emission maximum 461 nm) when bound to double stranded DNA datasheet is useful as a marker of nuclei for cell cycle studies to distinguish nuclear morphology in. Hoechst 33342 datasheet. Hoechst 33342 emits blue ﬂ uorescence when bound to double stranded DNA.It is slightly more membrane per- hoechst meant than the Hoechst 33258 analog. Hoechst 33342 is a cell- permeable fluorescent compound ( MW 561. Replicating or dividing chromosomes will not hoechst stain with Hoechst 33342 DNA dye. Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high- quality solution of Hoechst dye for fixed- live- cell fluorescent staining of DNA nuclei in cellular imaging techniques. Each vial of Hoechst 33342 contains 1 mL of aqueous solution at 200 μg/ mL ( catalog # 639). Assignment of DNA binding sites for 4' 6- diamidine- 2- phenylindole bisbenzimide ( Hoechst datasheet 33258). , 1988; 949: 158 ; Simultaneous quantitation of Hoechst 33342 and hoechst immunofluorescence on viable cells using a fluorescence activated cell sorter.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. Tocris products are intended for laboratory research use only, unless stated otherwise. Hoechst 33342 nucleic acid stain is a popular cell- permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA.
hoechst 33342 datasheet
This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. Hoechst 33342 trihydrochloride is an A: T- specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm.